منابع مشابه
Peptide Sequencing by Edman Degradation
By the middle of the twentieth century it was known that proteins were composed of amino acids, but not how these were joined together. Were they arranged in blocks of similar residues? or randomly mixed together? or in repeated patterns? At about that time, methods were devised to provide an answer to these questions: namely, that each individual protein has its constituent amino acid residues...
متن کاملSolving peptide sequencing as satisfiability
This paper presents an approach for determining the amino acid sequence of a peptide through the solution of propositional satisfiability problems. Data obtained from the mass spectrometry analysis of a peptide are used to build a propositional logic formula, whose models represent coherent interpretations of that set of data and can be used to generate all possible correct results of the analy...
متن کاملTwisted amide electrophiles enable cyclic peptide sequencing.
There is an ever-increasing interest in synthetic methods that not only enable peptide macrocyclization, but also facilitate downstream application of the synthesized molecules. We have found that aziridine amides are stereoelectronically attenuated in a macrocyclic environment such that non-specific interactions with biological nucleophiles are reduced or even shut down. The electrophilic reac...
متن کاملDe novo peptide sequencing by deep learning.
De novo peptide sequencing from tandem MS data is the key technology in proteomics for the characterization of proteins, especially for new sequences, such as mAbs. In this study, we propose a deep neural network model, DeepNovo, for de novo peptide sequencing. DeepNovo architecture combines recent advances in convolutional neural networks and recurrent neural networks to learn features of tand...
متن کاملPepNovo: de novo peptide sequencing via probabilistic network modeling.
We present a novel scoring method for de novo interpretation of peptides from tandem mass spectrometry data. Our scoring method uses a probabilistic network whose structure reflects the chemical and physical rules that govern the peptide fragmentation. We use a likelihood ratio hypothesis test to determine whether the peaks observed in the mass spectrum are more likely to have been produced und...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2012
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-012-6192-3